Engineering DNAzyme cascade for signal transduction and amplification
Date:2021-08-24 clicks:
Impact Factor:4.616
DOI number:10.1039/c9an02003a
Affiliation of Author(s):Hunan University
Journal:Analyst
Funded by:National Natural Science Foundation of China
Key Words:Inspired by the natural enzyme cascade reaction, a multiple DNAzyme cascade platform is engineered to imitate the intracellular process of signal transduction and signal amplification. In this design, when particular stimuli appear, an activated upstream DNAzyme will cleave a well-designed intermediary S1, releasing a downstream DNAzyme that can cleave the reporter substrate S2 to output signals. Thus, the signal is passed from the upstream DNAzyme to the downstream DNAzyme through a well-designed intermediary, accomplishing signal transduction and signal amplification. According to the experimental results, the DNAzyme cascades are capable of improving sensitivity for bioassays compared with that for single DNAzyme-based biocatalysis, which holds promise for potential applications, such as biomolecular computing, logic circuits and precision medicine.
Abstract:Inspired by the natural enzyme cascade reaction, a multiple DNAzyme cascade platform is engineered to imitate the intracellular process of signal transduction and signal amplification. In this design, when particular stimuli appear, an activated upstream DNAzyme will cleave a well-designed intermediary S1, releasing a downstream DNAzyme that can cleave the reporter substrate S2 to output signals. Thus, the signal is passed from the upstream DNAzyme to the downstream DNAzyme through a well-designed intermediary, accomplishing signal transduction and signal amplification. According to the experimental results, the DNAzyme cascades are capable of improving sensitivity for bioassays compared with that for single DNAzyme-based biocatalysis, which holds promise for potential applications, such as biomolecular computing, logic circuits and precision medicine.
First Author:Jing Li,‡ Ke Quan,‡ Yanjing Yang, Xiaohai Yang, Xiangxian Meng, Jin Huang* and Kemin Wang*
Indexed by:Other
Discipline:Natural Science
First-Level Discipline:Chemistry
Volume:145
Issue:5
Page Number:1925-1932
Translation or Not:no
Date of Publication:2020-01-20
Included Journals:SCI
Links to published journals:https://pubs.rsc.org/en/content/articlelanding/2020/an/c9an02003a
