Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells
Date:2021-08-24 clicks:
DOI number:10.7150/ntno.22960
Affiliation of Author(s):Hunan University
Journal:Nanotheranostics
Funded by:National Natural Science Foundation of China
Key Words:living cells, multiplexed microRNAs imaging, two-color-based nanoflares
Abstract:MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide critical information for our better understanding of their roles in cells and further validating of their function in clinical diagnoses. Simultaneous detection of multiple biomarkers could effectively improve the accuracy of early cancer diagnosis. Here, we develop the two-color-based nanoflares for simultaneously detecting two distinct miRNA targets inside live cells. The nanoflares consist of gold nanoparticles (AuNPs) functionalized with a dense shell of recognition sequences hybridized to two short fluorophore-labeled DNA molecules, termed “flares”. In this conformation, the close proximity of the fluorophore to the AuNPs surface leads to quenching of the fluorescence. However, when target miRNAs bind to the recognition sequence, the concomitant displacement of the flare can be detected as a corresponding increase in fluorescence. The results demonstrate that the two-color-based nanoflares can simultaneously detect miR-21 and miR-141 expression levels in various live cancer cells successfully. Compared to the traditional single-color-based nanoflares, the two-color-based nanoflares could offer more reliable and practical information for cancer detection, improving the accuracy of early disease diagnosis.
First Author:Jing Li, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Yanan Wu, Changbei Ma and Kemin Wang*
Indexed by:Other
Discipline:Natural Science
First-Level Discipline:Chemistry
Volume:2
Issue:1
Page Number:96-105
Translation or Not:no
Date of Publication:2018-01-01
Links to published journals:https://www.ntno.org/v02p0096.htm
