Orderly Assembled, Self-Powered FRET Flares for MicroRNA Imaging in Live Cells
影响因子：6.986
DOI码：10.1021/acs.analchem.1c00873
所属单位：Hunan University
发表刊物：Analytical Chemistry
项目来源：National Natural Science Foundation of China (NSFC)
关键字：DNA assembly, self-powered, FRET Flares, microRNA imaging, living cells
摘要：Signal amplification provides unparalleled opportunities for visualizing low-abundance targets in living cells. However, self-powered signal amplification has not been achieved because of the lack of "fuel" in living cells. Thus, the aim of this work was to develop an integrated amplification platform for the detection of intracellular miRNA by itself. This system, termed self-powered FRET flares (SPF), was first established by self-assembly to form a DNA nanostructure, and then the FRET flares and fuel DNA as the driving force were precisely and orderly loaded on it, which was able to power target recycle and realize signal amplification without any auxiliary additives under the trigger of intracellular miRNA-21. In addition, it employed AS1411 aptamer to target specific cancer cells and facilitated cell internalization of assembly DNA nanostructures. As a proof of concept, we demonstrated that SPF enabled rapid response to miRNA-21 and improvement of the detection sensitivity compared to previously proposed FRET flares without amplification. This strategy is promising for advancing integrated and self-powered nanomachines to execute diverse tasks, facilitating their biological and medical application.
第一作者：Jing Li,† Anmin Wang,† Xiaohai Yang, Kemin Wang, and Jin Huang*
论文类型：其它
学科门类：理学
一级学科：化学
卷号：93
期号：15
页面范围：6270-6277
是否译文：否
发表时间：2021-04-06
收录刊物：SCI
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