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影响因子：14.9

所属单位：Hunan University

发表刊物：Nucleic Acids Research

项目来源：National Natural Science Foundation of China

摘要：There is considerable interest in creating a precise and sensitive strategy for in situ visualizing and profiling intracellular miRNA. Present here is a novel photocaged amplified FRET nanoflare (PAFN), which spatiotemporal controls of mRNA-powered nanomachine for precise and sensitive miRNA imaging in live cells. The PAFN could be activated remotely by light, be triggered by specific low-abundance miRNA and fueled by high-abundance mRNA. It offers high spatiotemporal control over the initial activity of nanomachine at desirable time and site, and a ‘one-to-more’ ratiometric signal amplification model. The PAFN, an unprecedented design, is quiescent during the delivery process. However, upon reaching the interest tumor site, it can be selectively activated by light, and then be triggered by specific miRNA, avoiding undesirable early activation and reducing nonspecific signals, allowing precise and sensitive detection of specific miRNA in live cells. This strategy may open new avenues for creating spatiotemporally controllable and endogenous molecule-powered nanomachine, facilitating application at biological and medical imaging.

第一作者：Jing Li, Shiyuan Liu, Jiaoli Wang, Ruiting Liu, Xiaohai Yang, Kemin Wang, Jin Huang*

论文类型：SCI

卷号：50(7):

页面范围：e40

ISSN号：0305-1048

是否译文：否

发表时间：2022-01-01

收录刊物：SCI