Impact Factor:1.142
Journal:J Vet Sci
Key Words:1α,25-(OH)₂D₃; MMP-9; TRAP; bone lacunar resorption; osteoclast
Abstract:To investigate 1α,25-(OH)₂D₃ regulation of matrix metalloproteinase-9 (MMP-9) protein expression during osteoclast formation and differentiation, receptor activator of nuclear factor kB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were administered to induce the differentiation of RAW264.7 cells into osteoclasts. The cells were incubated with different concentrations of 1α,25-(OH)₂D₃ during culturing, and cell proliferation was measured using the methylthiazol tetrazolium method. Osteoclast formation was confirmed using tartrate-resistant acid phosphatase (TRAP) staining and assessing bone lacunar resorption. MMP-9 protein expression levels were measured with Western blotting. We showed that 1α,25-(OH)₂D₃ inhibited RAW264.7 cell proliferation induced by RANKL and M-CSF, increased the numbers of TRAP-positive osteoclasts and their nuclei, enhanced osteoclast bone resorption, and promoted MMP-9 protein expression in a concentration-dependent manner. These findings indicate that 1α,25-(OH)₂D₃ administered at a physiological relevant concentration promoted osteoclast formation and could regulate osteoclast bone metabolism by increasing MMP-9 protein expression during osteoclast differentiation.
First Author:Jian-Hong Gu, Xi-Shuai Tong, Guo-Hong Chen, Xue-Zhong Liu, Jian-Chun Bian, Yan Yuan, Zong-Ping Liu
Indexed by:SCI、
Volume:15
Issue:1
Page Number:133-140
Translation or Not:no
Date of Publication:2014-03-01
Included Journals:SCI