Impact Factor:3.88
DOI number:10.1016/j.diff.2021.08.003
Journal:Differentiation
Key Words:Autophagy; Beclin1; Osteoclastogenesis; Osteoprotegerin (OPG)
Abstract:Osteoclastogenesis is induced by receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF), and can be suppressed by osteoprotegerin (OPG). Beclin1 has a dual role in osteoclastogenesis. However, the role of Beclin1-mediated autophagy during OPG-induced inhibition of osteoclastogenesis remains unclear. Here, we found that Beclin1 and matrix metalloproteinase 9 (MMP-9) expression were increased during osteoclastogenesis. OPG (20, 40, and 80 ng/mL) decreased Src and MMP-9 expression, but augmented Beclin1 expression and fluorescence intensity. Similarly, treatment with the autophagy activator rapamycin increased Beclin1 expression during OPG-induced inhibition of osteoclastogenesis. Further, Beclin1 knockdown restored osteoclast numbers by reducing autophagy during OPG-induced inhibition of osteoclastogenesis. These results indicate that Beclin1 has a positive role during OPG-induced inhibition of osteoclastogenesis by regulating autophagy, which might provide a potential basis for osteoclastogenesis.
First Author:Xishuai Tong, Wenyan Min, Saihui Li, Miaomiao Chen, Ruilong Song, Jianchun Bian, Jianhong Gu, Zongping Liu
Indexed by:SCI
Volume:121:
Page Number:35-43
Translation or Not:no
Date of Publication:2021-08-23