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影响因子：3.88

DOI码：10.1016/j.diff.2021.08.003

发表刊物：Differentiation

关键字：Autophagy; Beclin1; Osteoclastogenesis; Osteoprotegerin (OPG)

摘要：Osteoclastogenesis is induced by receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF), and can be suppressed by osteoprotegerin (OPG). Beclin1 has a dual role in osteoclastogenesis. However, the role of Beclin1-mediated autophagy during OPG-induced inhibition of osteoclastogenesis remains unclear. Here, we found that Beclin1 and matrix metalloproteinase 9 (MMP-9) expression were increased during osteoclastogenesis. OPG (20, 40, and 80 ng/mL) decreased Src and MMP-9 expression, but augmented Beclin1 expression and fluorescence intensity. Similarly, treatment with the autophagy activator rapamycin increased Beclin1 expression during OPG-induced inhibition of osteoclastogenesis. Further, Beclin1 knockdown restored osteoclast numbers by reducing autophagy during OPG-induced inhibition of osteoclastogenesis. These results indicate that Beclin1 has a positive role during OPG-induced inhibition of osteoclastogenesis by regulating autophagy, which might provide a potential basis for osteoclastogenesis.

第一作者：Xishuai Tong, Wenyan Min, Saihui Li, Miaomiao Chen, Ruilong Song, Jianchun Bian, Jianhong Gu, Zongping Liu

论文类型：SCI

卷号：121:

页面范围：35-43

是否译文：否

发表时间：2021-08-23