一、个人简介：

   吴健，1986年生，博士，副教授，博士生导师，生物技术系主任。江苏省杰出青年基金获得者，国家重点研发计划青年科学家项目主持人，江苏省高校“青蓝工程”优秀青年骨干教师、扬州大学高端人才支持计划“拔尖人才成长计划”培养对象。主讲本科生《分子生物学（双语）》、《普通生物学》和研究生《植物研究技术》等课程。主要从事油菜抗病分子遗传以及基因组编辑研究，以第一或通讯作者（含共同）在Nature Communications、Plant Physiology（2022/2025）、Plant Biotechnology Journal（2019/2020/2024）、Plant Communications、Journal of Experimental Botany（2022/2025a/2025b）、Theoretical and Applied Genetics和Crop Journal（2019/2022）等Top期刊发表论文。累积引用次数为2000余次，H指数22。登记油菜新品种 1 个，授权发明专利 4 项。先后主持国家自然科学基金3项、江苏省农业自主创新项目2项、参加国家重点研发计划1项。

二、代表论文：

(1)         Liu DX^(#), Fan JL^(#), Ye Y, Liu Y, Ren SC. Lei WJ, Zhang XR, He AC, Xing JQ, Sun QF, Lin L, Wang YP, Wu J^(*). The transcription factor BnaWRKY75 enhances resistance to the necrotrophic pathogen Sclerotinia sclerotiorum by promoting salicylic acid biosynthesis in oilseed rape. Journal of Experimental Botany, 2025, eraf332 (通讯作者)

(2)         Lin C^(*), Shen XW, Zhu TT, Liu DX, Zhang ZD, Zhang Z, Long YX, Zhang JH, Sun QF, Dun XL, Wu J^(*), Wang YP^(*). BnaA07.WRKY40 transcription factor confers enhanced defensive response to Sclerotinia sclerotiorum in Brassica napus. Journal of Experimental Botany, 2025, eraf310 (共同通讯作者).

(3)         Wu J^(*), Xu JY, He AC, Ren SC, Ye Y, Lei WJ, Liu Y, Hua X, Wei CJ, Lin L, Zhang H, Wang YP^(*). Generating fragrant oilseed rape using CRISPR/Cas9-mediated gene editing. Plant Physiology, 2025, 197 (1), kiae660 (第一作者和共同通讯作者).

(4)         Lin L, Zhang XR, Fan JL, Li JW, Ren SC, Gu X, Li PP, Xu ML, Xu JY, Lei WJ, Liu DX, Sun QF, Cai GQ, Yang Q-Y, Wang YP^(*), Wu J^(*). Natural variation in BnaA07.MKK9 confers resistance to Sclerotinia stem rot in oilseed rape. Nature Communications, 2024, 15:5059 (共同通讯作者).

(5)         Sun CM^(#), Wu J^(#), Zhou XY, Fu SX, Liu HM, Xue ZF, Wang XD, Peng Q, Gao JQ, Chen F, Zhang W, Hu ML, Fu TD, Wang YP^(*), Yi B^(*), Zhang JF^(*). Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis. Plant Biotechnology Journal, 2024, 22(6):1636-1648 (共同第一作者)

(6)         Zhao Q, Wu J^(*), Lai L, Shahid M, Qasim MU, Yu KD, Zhang CY, Fan CC, Zhou YM^(*). Fine mapping and candidate gene analysis of a major QTL for oil content in the seed of Brassica napus. Theoretical and Applied Genetics, 2023, 136:256 (共同通讯作者)

(7)         Liu DX, Li JW, Wang SB, Huang TT, Tao FT, Lin YC, Lin W, Zhao XL, Huang YM, Jia YP, Yang ZQ, Luo CF, Zhu Q, Sung W-K, Wu J^(*), Yang Q-Y^(*). PlantCFG: A comprehensive database with web tools for analyzing candidate flowering genes in multiple plants. Plant Communications, 2024, 5:100733 (共同通讯作者)

(8)         Wu J, Liu HM, Ren SC, Li PP, Li X, Lin L, Sun QF, Zhang L. Lin C, Wang YP^(*). Generating an oilseed rape mutant with non-abscising floral organs using CRISPR/Cas9 technology. Plant Physiology, 2022, 190 (3), 1562-1565 (第一作者)

(9)         Lin L, Fan JL, Li PP, Liu DX, Ren SC, Lin KY, Fang YJ, Lin C, Wang YP^(*), Wu J^(*). The Sclerotinia sclerotiorum-inducible promoter pBnGH17^D7 in Brassica napus: isolation, characterization and application in host-induced gene silencing. Journal of Experimental Botany, 2022, 73(19): 6663-6677 (共同通讯作者)

(10)      Wu J^(*), Yin SL, Lin L, Liu DX, Ren SC, Zhang WJ, Meng WC, Chen PP, Sun QF, Fang YJ, Wei CX, Wang YP^(*). Host-induced gene silencing of multiple pathogenic factors of Sclerotinia sclerotiorum confers resistance to Sclerotinia rot in Brassica napus. The Crop Journal, 2022, 10(3): 661-671 (第一作者和共同通讯作者)

(11)     Wu J, Chen C, Xian GY, Liu DX, Lin L, Yin SL, Sun QF, Fang YJ, Zhang H^(*), Wang YP^(*). Engineering herbicide-resistant oilseed rape by CRISPR/Cas9-mediated cytosine base-editing. Plant Biotechnology Journal, 2020, 18(9):1857-1859 (第一作者)

(12)     Zhao Q^(#), Wu J^(#), Cai GQ, Yang QY, Shahid M, Fan CC, Zhang CY, Zhou YM^(*). A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus. Plant Biotechnology Journal, 2019, 17 (12):2313-2324 (共同第一作者)

(13)     Wu J, Chen PP, Zhao Q, Cai GQ, Hu Y, Xiang Y, Yang QY, Wang YP, Zhou YM^(*). Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus. The Crop Journal, 2019, 7:227-237 (第一作者)

(14)     Liu DX, Wu J^(*), Lin L, Li PP, Li SF, Wang Y, Li J, Sun QF, Liang JS, Wang YP^(*). Overexpression of cinnamoyl-CoA reductase in Brassica napus increases resistance to Sclerotinia sclerotiorum by affecting lignin biosynthesis. Frontiers in Plant Science, 2021, 12:732733 (共同通讯作者)

(15)     Lin L, Wu J^(*), Jiang MY, Wang YP^(*). Plant mitogen-activated protein kinase cascades in environmental stresses. International Journal of Molecular Science, 2021, 22:1543 (共同通讯作者)

(16)     Hu HZ^(#), Tang YW^(#), Wu J^(#), Chen FZ, Yang YD, Pan XC, Dong X, Jin XD, Liu S, Du XZ^(*). Brassica napus mediator subunit16 induces BnMED25-and BnWRKY33-activated defense signaling to confer Sclerotinia sclerotiorum resistance. Frontiers in Plant Science, 2021, 12: 663536 (共同第一作者)

(17)     Wu J, Lin L, Xu ML, Chen PP, Liu DX, Sun QF, Ran LP, Wang YP^(*).  Homoeolog expression bias and expression level dominance in resynthesized allopolyploid Brassica napus. BMC Genomics, 2018, 19(1):586 (第一作者)

(18)     Sun QF, Lin L, Liu DX, Wu DW, Fang YJ, Wu J^(*), Wang YP^(*). CRISPR/Cas9-Mediated Multiplex Genome Editing of the BnWRKY11 and BnWRKY70 Genes in Brassica napus L. International Journal of Molecular Sciences, 2018, 19 (9):2716 (共同通讯作者)

(19)     Wu J, Zhao Q, Yang QY, Liu H, Li QY, Yi XQ, Cheng Y, Guo L, Fan CC, Zhou YM^(*). Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus. Scientific Reports, 2016, 6:19007 (第一作者)

(20)     Wu J^(#), Zhao Q^(#), Liu S^(#), Shahid M, Lan L, Cai GQ, Zhang CY, Fan CC, Wang YP, Zhou YM^(*). Genome-wide association study identifies new loci for resistance to Sclerotinia stem rot in Brassica napus. Frontiers in Plant Science, 2016, 7:1418 (共同第一作者)

(21)     Wu J, Cai GQ, Tu JY, Li LX, Liu S, Luo XP, Zhou LP, Fan CC, Zhou YM^(*). Identification of QTLs for resistance to Sclerotinia stem rot and BnaC. IGMT5. a as a candidate gene of the major resistant QTL SRC6 in Brassica napus. Plos One, 2013, 8 (7):e67740 (第一作者)

(22)     吴健, 周永明, 王幼平^(*). 油菜与核盘菌互作分子机理研究进展. 中国油料作物学报, 2018, 40(5):721-729 (第一作者)

(23)     吴健, 涂江颖, 周永明^(*). 甘蓝型油菜抗菌核病研究进展. 中国油料作物学报, 2013, 35(5):608-615 (第一作者)

三、授权专利

(1) 吴健, 王幼平, 林俐, 孙勤富, 刘东晓, 方玉洁, 蒋金金, 吴德伟, 一种受核盘菌诱导的油菜启动子pBnGH、鉴定方法和应用, 专利号：ZL201811570132.2.

(2) 吴健, 霍诗诗, 王幼平, 张辉, 倪萍, 基于基因编辑的甘蓝型油菜BnALS1突变型基因、蛋白及其应用, 专利号：ZL202010051263.0.

(3) 吴健, 林俐, 钱沅澎, 黄雯婧, 顿小玲, 王幼平, BnaA07.MKK9基因或其SNAP分子标记在调控植物菌核病抗性中的应用, 专利号：ZL202410124874.1.