扬州大学教师个人主页服务平台袁玉国--中文主页-- Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland

袁玉国

学位：博士
职称：副高级
所在单位：兽医学院

博士生导师

硕士生导师

教师英文名称：Yuguo Yuan
教师拼音名称：Yuan Yuguo
电子邮箱：
入职时间：2010-06-01
学历：博士研究生毕业
办公地点：扬州大学文汇路校区45楼102室
性别：男
联系方式：0514-87979228
在职信息：在岗
毕业院校：扬州大学

学科：临床兽医学

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Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland

点击次数：

影响因子：

2.8

发表刊物：

Biosci Rep

摘要：

Poor expression is the key factor hampering the large-scale application of transgenic animal mammary gland bioreactors. A very different approach would be to evaluate the secretion of recombinant proteins into milk in response to a cleavable signal peptide of highly secreted lactoproteins.We previously reported rabbits harboring mammary gland-specific expression vector containing a fusion cDNA (goat β-lactoglobulin (BLG) signal peptide and recombinant human plasminogen activator (rhPA) coding sequences) expressed rhPA in the milk, but we did not realize the signal peptide contributed to the high rhPA concentration and did not mention it at that time. And the molecular structure and biological characteristics still remain unknown. So, rhPA in the milk was purified and characterized in the present study.rhPA was purified from the milk, and the purity of the recovered product was 98% with no loss of biological activity. Analysis of the N-terminal sequence, C-terminal sequence, and the molecular mass of purified rhPA revealed that they matched the theoretical design requirements. The active systemic anaphylaxis (ASA) reactions of the purified rhPA were negative. Taken together, these results indicated that the goat BLG signal peptide can efficiently mediate rhPA secretion into milk and was accurately cleaved off from rhPA by endogenous rabbit signal peptidase.We have reinforced the importance of a rhPA coding region fused to a cleavable heterologous signal peptide from highly secreted goat BLG to improve recombinant protein expression. It is anticipated that these findings will be widely applied to high-yield production of medically important recombinant proteins.

论文类型：

Research Atricle

文献类型：

Research Atricle

是否译文：

否

发表时间：

2019-06-12

收录刊物：

SCI

发布期刊链接：

https://pubmed.ncbi.nlm.nih.gov/31196965/

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个人简介

袁玉国，博士，副教授，硕士、博士生导师。2010年毕业于扬州大学兽医学院，获临床兽医学博士学位，并留校任教。2015.10—2017.7韩国Konkuk University博士后，2017.9—2018.9韩国Gyeongsang National University访问学者。

长期从事动物体细胞克隆和转基因克隆的研究工作，2007年在国内外首次报道利用成年乳腺上皮细胞获得克隆山羊，先后获得转人乳铁蛋白，白蛋白等转基因克隆羊。利用TALEN，Cas9技术获得Fel d1基因敲除猫、MSTN基因敲除山羊，人高血脂兔动物模型（兔）等。同时开展了功能纳米材料的制备及其在抗菌、抗肿瘤和化妆品上的应用。先后主持了国家自然科学基金、国家转基因重大专项子课题、江苏省种业“揭榜挂帅”等15余项课题的研究工作，在 EBioMedicine，Int J Nanomedicine，Cells，Oxid Med Cell Longev, Int J Mol Sci，Mol Reprod Dev，Theriogenology等杂志发表SCI论文30余篇，获得省部级等科技奖励2项、发明专利1项。

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