Impact Factor:2.819
DOI number:10.1016/j.virol.2019.12.006
Affiliation of Author(s):扬州大学
Journal:Virology
Key Words:Classical swine fever virus；NS5A；Unfolded protein response； GRP78
Abstract:Classical swine fever is a world organization for animal health listed disease and is caused by classical swine fever virus (CSFV). CSFV can induced unfolded protein response (UPR) and whether NS5A protein plays a role in this process remains unknown. Here, we demonstrate that CSFV induced all the three signal pathways ATF6, IRE1 and PERK of UPR. Furthermore, this phenomenon may be mediated by the NS5A protein since expression of NS5A alone can achieve the same effect. In the current study, we show that NS5A can interact with GRP78 as measured by using the CO-IP and GST pulldown assays. This interaction plays a positive role in the promotion of CSFV replication. Overexpression or knockdown of GRP78 mediated by lentivirus can enhance or decrease viral replication, respectively. Our findings provide the evidence that CSFV infection can activate the cellular UPRs, in which NS5A and GRP78 play key roles in the process.
Co-author:Zhao Fuxi,Guo Mengjiao,Ruan Baoyang,Wang Xuefeng,Wu Yantao
First Author:Zhang Chengcheng
Indexed by:Unit Twenty Basic Research
Correspondence Author:Zhang Xiaorong
Discipline:Agricultural science
First-Level Discipline:Veterinary Medicine
Document Type:J
Volume:541
Page Number:75-84
Translation or Not:no
Date of Publication:2020-02-15
Included Journals:SCI