扬州大学教师个人主页服务平台张小荣--中文主页-- CSFV protein NS5A activates the unfolded protein response to promote viral replication

论文成果

CSFV protein NS5A activates the unfolded protein response to promote viral replication

发布时间：2021-04-29点击次数：

影响因子：2.819
DOI码：10.1016/j.virol.2019.12.006
所属单位：扬州大学
发表刊物：Virology
关键字：Classical swine fever virus；NS5A；Unfolded protein response； GRP78
摘要：Classical swine fever is a world organization for animal health listed disease and is caused by classical swine fever virus (CSFV). CSFV can induced unfolded protein response (UPR) and whether NS5A protein plays a role in this process remains unknown. Here, we demonstrate that CSFV induced all the three signal pathways ATF6, IRE1 and PERK of UPR. Furthermore, this phenomenon may be mediated by the NS5A protein since expression of NS5A alone can achieve the same effect. In the current study, we show that NS5A can interact with GRP78 as measured by using the CO-IP and GST pulldown assays. This interaction plays a positive role in the promotion of CSFV replication. Overexpression or knockdown of GRP78 mediated by lentivirus can enhance or decrease viral replication, respectively. Our findings provide the evidence that CSFV infection can activate the cellular UPRs, in which NS5A and GRP78 play key roles in the process.
合写作者：赵富熙,郭梦娇,阮宝阳,王学峰,吴艳涛
第一作者：张成成
论文类型：基础研究
通讯作者：张小荣
学科门类：农学
一级学科：兽医学
文献类型：J
卷号：541
页面范围：75-84
是否译文：否
发表时间：2020-02-15
收录刊物：SCI

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