Haidong Ding
Gender:Male
Education Level:With Certificate of Graduation for Doctorate Study
Alma Mater:南京农业大学
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丁海东,男,2009年毕业于南京农业大学生命科学学院植物学专业获理学博士学位,同年进入扬州大学生物科学与技术学院工作,2015年博士后流动站出站。先后到德国马丁路德大学和日本名古屋大学访学。目前主要研究方向是果蔬(番茄)抗性生理与分子生物学。随着温室效应的加剧,全球气候持续升高,高温环境是影响番茄(Solanum lycopersicum Mill.)产量和品质的主要因素之一。因此,如何提高番茄的耐热能力是目前番茄育种研究的重要内容之一。通过现代生物技术手段挖掘抗性基因,解析其耐高温的分子机理、生理机制和信号转导机理研究是生命科学亟待解决的重大基础科学问题。我们通过转录组学、蛋白质组学、代谢组学等手段挖掘番茄高温应答基因,并通过基因工程手段(过表达、RNA干扰、CRISPR/Cas9基因编辑)获得该基因的过表达及干扰/敲除转基因株系来评估番茄叶片中高温诱导基因与热击蛋白(HSPs)表达、抗氧化损伤、光合作用及番茄耐热性的关系;进一步通过酵母双杂(Y2H)、荧光互补(BiFC)、Pull-down 等技术寻找其互作蛋白(靶蛋白),并对蛋白的功能进行研究。这一研究有利于我们理解番茄响应高温胁迫并调控生理耐热适应性的分子基础,对利用基因工程手段提高农业上蔬菜农作物耐热品种的选育和种植具有重要的指导意义。
先后主持国家自然科学基金项目2项(31101092、32172546)、江苏省自然科学基金1项(BK20191437)、中国博士后基金特别资助1项(2012T50493)、江苏省高校自然科学研究基金项目1项(10KJB180010)、参与国家自然科学基金3项(31101093、31201213、31271622)。在SCI源刊物上发表论文30多篇,主要发表在本学科国际学术SCI源期刊(Plant Physiology、New Phytologist、Journal of Experimental Botany、Planta、Environmental and Experimental Botany、Journal of Integrative Plant Biology)等。
目前主要承担《植物学》、《植物学实验》、《生物学野外实习》等课程的教学;先后也承担《普通生物学》、《生物安全学》和研究生《植物分子生物学》课程的教学;《植物学》课程获江苏省首批一流课程和国家一流课程(2021、2023);主编教材《植物学》(2023)《植物学实验与技术》(2022)和《阆苑芝萃》– 扬州大学校园植物图鉴(2019);作为副主编参编《植物学学习指南与题解》(2017);先后主持校级重点教改课题1项(2021)、校级卓越本科课程建设工程项目1项(2022)和校级精品本科教材建设工程项目建设项目1项(2022);先后获得扬州大学优秀混合课程(植物学,2021)、扬州大学本科教学成果奖二等奖(2022);第九、十届江苏省师范生教学基本功大赛优秀指导教师;扬州大学第三届教育硕士实践创新能力大赛优秀指导教师。
科研团队详情见下方
发表文章
Jiang, H., Liu, X., Xiao, P., Wang, Y., Xie, Q., Wu, X., Ding, H*. (2023). Functional insights of plant bcl-2–associated ahanogene (BAG) proteins: Multi-taskers in diverse cellular signal transduction pathways. Front. Plant Sci, 14, 1136873.(SCI)
Ding H, Xie Q, Ji Y, Qian L, Guan Y, Ge C. The Characteristics of Solanum lycopersicum SlSPRH1 and its Negative Role in Thermotolerance in Arabidopsis. Journal of Plant Growth Regulation, 2023, 42: 908–921.(SCI)
Wu X, Wan Y, Bian Y, Ren Y, Xu X, Zhou F, Ding H*. A critical review on plant annexin: Structure, function, and mechanism. Plant Physiology and Biochemistry 2022, 190, 81-89.
Jiang H, Ji Y, Sheng J, Wang Y, Liu X, Xiao P, Ding H*. Genome-wide identification of the Bcl-2 associated athanogene (BAG) gene family in Solanum lycopersicum and the functional role of SlBAG9 in response to osmotic stress. Antioxidants, 2022, 11, 598.(SCI)
Ding H, Qian L, Jiang H, Ji, Y, Fang Y, Sheng J, Xu X, Ge C. Overexpression of a Bcl-2-associated athanogene SlBAG9 negatively regulates high-temperature response in tomato. International Journal of Biological Macromolecules 2022, 194, 695-705.(SCI)
Ding H, Qian Y, Fang Y, Ji Y, Sheng J, Ge, C. Characteristics of SlCML39, a tomato calmodulin-like gene, and Its negative role in high temperature tolerance of Arabidopsis thaliana during germination and seedling growth. Int. J. Mol. Sci. 2021, 22, 11479.(SCI)
Yuan G, Qian Y, Ren Y, Guan Y, Wu X, Ge C, Ding H*. The role of plant-specifc VQ motif-containing proteins: An ever-thickening plot. Plant Physiology and Biochemistry, 2021, 159:12–16(SCI)
Mo S, Qian Y, Zhang W, Qian L, Wang Y, Cailin G, Ding H*. Mitogen-activated protein kinase action in plant response to high-temperature stress: a mini review. Protoplasma, 2021, 258:477–482(SCI)
Wu X, Ren Y, Jiang H, Wang Y, Yan J, Xu X, Zhou F, Ding H*. Genome-wide identification and transcriptional expression analysis of annexin genes in Capsicum annuum and characterization of CaAnn9 in salt tolerance. Int. J. Mol. Sci. 2021, 22, 8667.(SCI)
Ding H, Mo S, Qian Y, Yuan G, Wu, X, Ge, C. Integrated proteome and transcriptome analyses revealed key factors involved in tomato (solanum lycopersicum) under high temperature stress. Food and Energy Security. 2020;9:e239.(SCI)
Xu X, Chen Q, Mo S, Qian Y, Wu X, Jin Y, Ding H*. Transcriptome-wide modulation combined with morpho-physiological analyses of Typha orientalis roots in response to lead challenge. Journal of Hazardous Materials, 2020, 384:121405(SCI)
Ding H, Wu Y, Yuan G, Mo S, Chen Q, Xu X, Wu X, Ge C. In-depth proteome analysis reveals multiple pathways involved in tomato SlMPK1-mediated high-temperature responses. Protoplasma, 2020, 257:43–59.(SCI)
Wu X, Yan J, Wu Y, Zhang H, Mo S, Xu X, Zhou F, Ding H*. Proteomic analysis by iTRAQ-PRM provides integrated insight into mechanisms of resistance in pepper to Bemisia tabaci (Gennadius). BMC plant biology, 2019, 19(1), 270.(SCI)
Frank J, Happeck R, Meier B, Hoang MTT, Stribny J, Hause G, Ding H, Morsomme P, Baginsky S, Peiter, E. Chloroplast‐localized BICAT proteins shape stromal calcium signals and are required for efficient photosynthesis. New Phytologist, 2019, 221(2): 866-880(SCI)
Ding H, Yuan G, Mo S, Qian Y, Wu Y, Chen Q, Xu X, Wu X, Ge C. Genome-wide analysis of the plant-specific VQ motif-containing proteins in tomato (Solanum lycopersicum) and characterization of SlVQ6 in thermotolerance. Plant Physiology and Biochemistry, 2019, 143:29-39(SCI)
Ding H, He J, Wu Y, Wu X, Ge C, Wang Y, Zhong S, Peiter E, Liang J, Xu W. The tomato mitogen-activated protein kinase SlMPK1 is as a negative regulator of the high temperature stress response. Plant Physiology, 2018, 177: 633–651(SCI)
He J, Wang Y, Ding H*, Ge C. Epibrassinolide confers zinc stress tolerance by regulating antioxidant enzyme responses, osmolytes, and hormonal balance in Solanum melongena, seedlings. Brazilian Journal of Botany, 2016, 39(1):295-303. (SCI)
武媛, 何洁, 臧栋楠, 张吉, 杨帆, 朱晓红, 丁海东*. 酵母双杂交系统筛选番茄SlMPK1互作蛋白. 扬州大学学报(农业与生命科学版), 2019, 40(6): 24-29.(中文核心)
原贵波, 莫双榕, 钱莹, 臧栋楠, 杨帆, 蒋红亮, 武媛, 丁海东*. 应用GST pull-down技术筛选番茄SIVQ6互作蛋白. 中国农业科学, 2020, 53(15): 3146-3157.(中文核心)
丁海东, 钱莹, 谢其慧, 纪宇蓉, 刘丹, 蒋红亮. GST pull-down联合质谱分析筛选番茄SLSPRH1的互作蛋.扬州大学学报(农业与生命科学版), 2021, 42(06):36-41.(中文核心)
蒋海龙,盛佳容,黄孟娜,纪宇蓉,高欣怡,张文娟,丁海东*. 番茄SlBAG9基因启动子克隆及其对高温的应答. 分子植物育种,2022,网络首发.(中文核心)
[1]
南京农业大学
| 植物学
| 博士
| With Certificate of Graduation for Doctorate Study
[2] 2002.9 to 2005.6
扬州大学
| 生物物理学
| Master's Degree in Science
| 全日制学术型硕士
[3] 1998.9 to 2002.6
扬州大学
| Agricultural science
| Bachelor's Degree in Agriculture
| Undergraduate (Bachelor’s degree)
[1] 2018.12 to 2019.11
日本名古屋大学(Nagoya University)
| 访问学者
[2] 2014.2 to 2015.1
德国马丁-路德大学(Martin-Luther-Universität-Halle-Wittenberg)
| 访问学者
[3] 2010.8 to 2015.12
扬州大学
| 农学院作物学博士后流动站
| 博士后
[4] 2009.6 to Now
扬州大学
| 生物科学与技术学院
| 在职