Amplified FRET Nanoflares: An Endogenous mRNA-Powered Nanomachine for Intracellular MicroRNA Imaging
Date:2021-08-23 clicks:
Impact Factor:15.336
DOI number:10.1002/anie.202008245
Affiliation of Author(s):Hunan University
Journal:Angewandte Chemie International Edition
Funded by:National Natural Science Foundation of China (NSFC)
Key Words:amplified FRETnanoflares, intracellular imaging, microRNA, mRNA, nanomachines
Abstract:It is of great value to detect biological molecules in live cells. However, probes for imaging low-abundance targets in live cells are limited by the one-to-one signal-triggered model. Here, we introduce the concept of the amplified FRET nanoflare, which employs high-abundance endogenous mRNA as fuel strands to amplify the detection of low abundance intracellular miRNA. As far as we know, this is the first report of an endogenous mRNA-powered nanomachine for intracellular molecular detection. We experimentally prove the mechanism of the nanomachine and demonstrate its specificity and sensitivity. The proposed amplified FRET nanoflare can act as an excellent intracellular molecular detection strategy that is promising for biological and medical applications.
First Author:Jing Li, Jiaoli Wang, Shiyuan Liu, Nuli Xie, Ke Quan, Yanjing Yang, Xiaohai Yang, Jin Huang* and Kemin Wang*
Indexed by:Research Article
Discipline:Natural Science
First-Level Discipline:Chemistry
Volume:59
Issue:45
Page Number:20104-20111
Translation or Not:no
Date of Publication:2020-07-28
Included Journals:SCI
Links to published journals:https://onlinelibrary.wiley.com/doi/10.1002/anie.202008245
