Orderly-assembled, self-powered FRET flares for microRNA imaging in live cells
Date:2021-08-23 clicks:
Impact Factor:6.986
DOI number:10.1021/acs.analchem.1c00873
Affiliation of Author(s):Hunan University
Journal:Analytical Chemistry
Funded by:National Natural Science Foundation of China (NSFC)
Key Words:DNA assembly, self-powered, FRET Flares, microRNA imaging, living cells
Abstract:Signal amplification provides unparalleled opportunities for visualizing low-abundance targets in living cells. However, self-powered signal amplification has not been achieved because of the lack of "fuel" in living cells. Thus, the aim of this work was to develop an integrated amplification platform for the detection of intracellular miRNA by itself. This system, termed self-powered FRET flares (SPF), was first established by self-assembly to form a DNA nanostructure, and then the FRET flares and fuel DNA as the driving force were precisely and orderly loaded on it, which was able to power target recycle and realize signal amplification without any auxiliary additives under the trigger of intracellular miRNA-21. In addition, it employed AS1411 aptamer to target specific cancer cells and facilitated cell internalization of assembly DNA nanostructures. As a proof of concept, we demonstrated that SPF enabled rapid response to miRNA-21 and improvement of the detection sensitivity compared to previously proposed FRET flares without amplification. This strategy is promising for advancing integrated and self-powered nanomachines to execute diverse tasks, facilitating their biological and medical application.
First Author:Jing Li,† Anmin Wang,† Xiaohai Yang, Kemin Wang, and Jin Huang*
Indexed by:SCI
Discipline:Natural Science
First-Level Discipline:Chemistry
Volume:93
Issue:15
Page Number:6270-6277
Translation or Not:no
Date of Publication:2021-04-06
Included Journals:SCI
Links to published journals:https://pubs.acs.org/doi/10.1021/acs.analchem.1c00873
