点击次数：

影响因子：15.336

DOI码：10.1002/anie.202008245

所属单位：Hunan University

发表刊物：Angewandte Chemie International Edition

项目来源：National Natural Science Foundation of China (NSFC)

关键字：amplified FRET nanoflares, intracellular imaging, microRNA, mRNA, nanomachine

摘要：It is of great value to detect biological molecules in live cells. However, probes for imaging low-abundance targets in live cells are limited by the one-to-one signal-triggered model. Here, we introduce the concept of the amplified FRET nanoflare, which employs high-abundance endogenous mRNA as fuel strands to amplify the detection of low abundance intracellular miRNA. As far as we know, this is the first report of an endogenous mRNA-powered nanomachine for intracellular molecular detection. We experimentally prove the mechanism of the nanomachine and demonstrate its specificity and sensitivity. The proposed amplified FRET nanoflare can act as an excellent intracellular molecular detection strategy that is promising for biological and medical applications.

第一作者：Jing Li, Jiaoli Wang, Shiyuan Liu, Nuli Xie, Ke Quan, Yanjing Yang, Xiaohai Yang, Jin Huang* and Kemin Wang*

论文类型：其它

学科门类：理学

一级学科：化学

卷号：59

期号：45

页面范围：20104-20111

是否译文：否

发表时间：2020-07-28

收录刊物：SCI

发布期刊链接：https://onlinelibrary.wiley.com/doi/10.1002/anie.202008245