Engineering DNAzyme Cascade for Signal Transduction and Amplification
影响因子：4.616
DOI码：10.1039/c9an02003a
所属单位：Hunan University
发表刊物：Analyst
项目来源：National Natural Science Foundation of China
关键字：signal amplification, DNAzyme cascade
摘要：Inspired by the natural enzyme cascade reaction, a multiple DNAzyme cascade platform is engineered to
imitate the intracellular process of signal transduction and signal amplification. In this design, when particular
stimuli appear, an activated upstream DNAzyme will cleave a well-designed intermediary S1, releasing
a downstream DNAzyme that can cleave the reporter substrate S2 to output signals. Thus, the signal is
passed from the upstream DNAzyme to the downstream DNAzyme through a well-designed intermediary,
accomplishing signal transduction and signal amplification. According to the experimental results, the
DNAzyme cascades are capable of improving sensitivity for bioassays compared with that for single
DNAzyme-based biocatalysis, which holds promise for potential applications, such as biomolecular computing,
logic circuits and precision medicine.
第一作者：Jing Li,‡ Ke Quan,‡ Yanjing Yang, Xiaohai Yang, Xiangxian Meng, Jin Huang* and Kemin Wang*
论文类型：其它
学科门类：理学
一级学科：化学
卷号：145
期号：5
页面范围：1925-1932
是否译文：否
发表时间：2020-01-20
收录刊物：SCI
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