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影响因子：4.616

DOI码：10.1039/c9an02003a

所属单位：Hunan University

发表刊物：Analyst

项目来源：National Natural Science Foundation of China

关键字：signal amplification, DNAzyme cascade

摘要：Inspired by the natural enzyme cascade reaction, a multiple DNAzyme cascade platform is engineered to imitate the intracellular process of signal transduction and signal amplification. In this design, when particular stimuli appear, an activated upstream DNAzyme will cleave a well-designed intermediary S1, releasing a downstream DNAzyme that can cleave the reporter substrate S2 to output signals. Thus, the signal is passed from the upstream DNAzyme to the downstream DNAzyme through a well-designed intermediary, accomplishing signal transduction and signal amplification. According to the experimental results, the DNAzyme cascades are capable of improving sensitivity for bioassays compared with that for single DNAzyme-based biocatalysis, which holds promise for potential applications, such as biomolecular computing, logic circuits and precision medicine.

第一作者：Jing Li,‡ Ke Quan,‡ Yanjing Yang, Xiaohai Yang, Xiangxian Meng, Jin Huang* and Kemin Wang*

论文类型：其它

学科门类：理学

一级学科：化学

卷号：145

期号：5

页面范围：1925-1932

是否译文：否

发表时间：2020-01-20

收录刊物：SCI

发布期刊链接：https://pubs.rsc.org/en/content/articlelanding/2020/an/c9an02003a