点击次数：

影响因子：6.057

DOI码：10.1016/j.talanta.2018.02.036

所属单位：Hunan University

发表刊物：Talanta

项目来源：National Natural Science Foundation of China (NSFC)

关键字：2 '-O-methyl modified DNA probes, Gold nanoparticleMiRNA-21, Breast cancer cells

摘要：MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulated diverse cellular processes including differentiation, proliferation, apoptosis, metabolism and signal transduction pathways. An increasing number of data suggested that miRNA-21 could be identified as diagnostic and therapeutic biomarker for breast cancer. Meanwhile, inhibiting the function of miRNA-21, resulting in cells growth inhibition and apoptotic cells death. To realize miRNA-2ldetection and inhibition to diagnostic and therapeutic breast cancer cells, we developed gold nanoparticle-based 2'-O-methyl modified DNA probes (AuNP-2'-OMe-DNA probes) for diagnostic and therapeutic breast cancer. Gold nanoparticles were functionalized with chemically modified miRNA-21 inhibitor to suppress the function of miRNA-21 for the therapeutic breast cancer, at the same time, fluorophore-labeled DNA molecules were hybridized with antimiRNA-21 for diagnostic breast cancer. The results showed that the 2'-O-methyl modified DNA can improve stability, increase binding affinity to target strands and enhance the therapeutic effects. The experimental results also demonstrated that antimiR-21 were efficiently introduced into the cells and knocked down miRNA-21 to inhibit its function, leading to growth inhibition and apoptotic cells death. We prospected that chemically modified miRNA-21 inhibitor based on gold nanoparticles would be as a promising diagnostic and therapeutic platform for breast cancer clinically.

第一作者：Jing Li, Jin Huang*, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Yanan Wu, Changbei Ma, Kemin Wang*

论文类型：其它

学科门类：理学

卷号：183

页面范围：11-17

是否译文：否

发表时间：2018-06-01

收录刊物：SCI

发布期刊链接：https://www.sciencedirect.com/science/article/pii/S0039914018301450?via%3Dihub