发表刊物：Frontiers in Plant Science
关键字：wheat, quality, mutant, HMW-GS, cookie
摘要：The area of middle to lower reaches of Yangtze River is the largest region for soft wheat production in China. In soft wheat breeding, lack of germplasm with desirable quality for end-use products is a barrier. Ningmai9 is the main variety of soft wheat planted in this area. In order to create the germplasm with better quality and yield potential than Ningmai9, mutants of HMW-GSs in Ningmai9 induced by ethylmethanesulfonate (EMS) were obtained. SDS-PAGE showed that two mutants, md10 and md11, were HMW-GS 1Dy deletions. DNA sequencing confirmed that one mutation was caused by a C/T substitution, resulting in the change of CAA encoding glutamine into the termination codon TAA, and another mutation is due to a G/A substitution in central repetitive domain of the coding region, causing TGG encoding tryptophan to become the termination codon TGA. Premature termination codon of 1Dy12 gene affected the expression of 1Dy12 and kept the mRNA at a lower transcription level during kernel development stage in comparison with the wild type. HMW-GS 1Dy12 deletion mutants decreased the content of HMW-GSs and glutenin macropolymers, mixograph envelope peak time and TIMEX width, water solvent retention capacity (WSRC) and lactic acid solvent retention capacity (LASRC). In the HMW-GS 1Dy12 deletion lines, sugar-snap cookie diameter was 8.70-8.74 cm, which was significantly larger than that in the wild type of 8.0 cm. There were no significant differences in spike number, kernel number, thousand kernel weight and yield between deletion lines and wild type. Overall, the study indicated that the knockout of HMW-GS gene induced by EMS is an effective way to improve wheat quality, and deletion mutants of HMW-GS 1Dy12 decrease gluten strength and increase sugar snap cookie diameter without yield penalty in Ningmai9 wheat.
论文类型：SCI
论文编号：835164
学科门类：农学
一级学科：作物学
文献类型：SCI
卷号：13
是否译文：否
发表时间：2022-05-12
收录刊物：SCI