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影响因子：3.448

DOI码：10.1002/jcb.27468

发表刊物：J Cell Biochem

关键字：70‐kDa ribosomal protein S6 kinase; AMP‐activated protein kinase; mammalian target of rapamycin; osteoclast; osteoprotegerin

摘要：Osteoclasts are highly differentiated terminal cells formed by fusion of hematopoietic stem cells. Previously, osteoprotegerin (OPG) inhibit osteoclast differentiation and bone resorption by blocking receptor activator of nuclear factor-κB ligand (RANKL) binding to RANK indirect mechanism. Furthermore, autophagy plays an important role during osteoclast differentiation and function. However, whether autophagy is involved in OPG-inhibited osteoclast formation and bone resorption is not known. To elucidate the role of autophagy in OPG-inhibited osteoclast differentiation and bone resorption, we used primary osteoclast derived from mice bone marrow monocytes/macrophages (BMM) by induced M-CSF and RANKL. The results showed that autophagy-related proteins expression were upregulated; tartrate-resistant acid phosphatase-positive osteoclast number and bone resorption activity were decreased; LC3 puncta and autophagosomes number were increased and activated AMPK/mTOR/p70S6K signaling pathway. In addition, chloroquine (as the autophagy/lysosome inhibitor, CQ) or rapamycin (as the autophagy/lysosome inhibitor, Rap) attenuated osteoclast differentiation and bone resorption activity by OPG treatment via AMPK/mTOR/p70S6K signaling pathway. Our data demonstrated that autophagy plays a critical role in OPG inhibiting osteoclast differentiation and bone resorption via AMPK/mTOR/p70S6K signaling pathway in vitro.

第一作者：Xishuai Tong, Jianhong Gu, Ruilong Song, Dong Wang, Ziqiang Sun, Chen Sui, Chuang Zhang, Xuezhong Liu, Jianchun Bian, Zongping Liu

论文类型：SCI、

是否译文：否

发表时间：2018-09-06

收录刊物：SCI