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发表刊物：J Steroid Biochem Mol Biol

关键字：1α,25-(OH)2D3Osteoclasts, Bone resorption, RANKL, OPG, RAW264.7 cells

摘要：The hormonally active form of vitamin D3, 1α,25-(OH)2D3, has an important role in bone metabolism. This study examined the effects of 1α,25-(OH)2D3 on the ability of two cytokines, receptor activator of nuclear factor-κB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF), to induce RAW 264.7 cells to form osteoclasts. A TRAP histochemical staining assay and bone resorption analysis were used to identify the rate of formation and activity of osteoclasts. The numbers of osteoclasts formed, and their bone resorption activity, was enhanced by the addition of 1α,25-(OH)2D3. The expression levels of osteoclast-specific proteins that are essential for bone resorption, integrin β3, V-ATPase, CAII, CTSK, TRAP and MMP-9, were detected by western blotting. During 48 h, the expression levels of all these proteins significantly increased. Quantitative real-time polymerase chain reaction was used to determine the expression levels of the transcription factors, c-Fos and NFATcl. The expression levels of c-Fos and NFATc1 also increased 24h after treatment with 1α,25-(OH)2D3. These results suggest that 1α,25-(OH)2D3 can regulate bone metabolism by directly enhancing the formation and maturation of osteoclasts.

第一作者：Jianhong Gu, Xi-Shuai Tong, Guo-Hong Chen, Dong Wang, Yang Chen, Yan Yuan, Xue-Zhong Liu, Jian-Chun Bian, Zong-Ping Liu

论文类型：SCI、

卷号：152

页面范围：25-33

是否译文：否

发表时间：2015-08-01