周昕，校特聘教授，研究员，博士生导师。

一、研究方向

1. 噬菌体探针可视化检测生物种族。

2. 基因工程化噬菌体抗菌。

3. 基于噬菌体的纳米疫苗。

二、主持项目

1. 国家重点研发项目子课题 “多重耐药菌噬菌体资源库构建及噬菌体与宿主菌互作机制研究”，2025YFC3408500，2025-08至2028-07，80万，在研。

2. 国家重点研发项目子课题“基于噬菌体展示技术的病原可视化现场快速检测技术研究”，2022YFC2604204，2022-12至2025-11，98.9万，在研。

3. 国家自然科学基金面上项目"短噬菌体SPR探针暗场快速、超灵敏、可视化检测沙门菌的研究"，32271435，2023-01至2026-12，54万，在研。

4. 上海市农科委，科技创新行动计划项目"基于噬菌体的H3N2亚型AIV快速检测技术研发"，2022-04至2025-03，80万，结题。

5. 国家自然科学基金面上项目"用于高灵敏、可视化病毒检测的噬菌粒纳米探针研究"，31870989，2019-01至2022-12，59万，结题。

6. 江苏省科技厅"双创团队"项目“猪重要病毒的可视化检测研究”，337090055，2018-10至2021-09，60万，结题。

7. 国家重点研发项目子课题“家禽致病性衣原体和沙门菌快速鉴定新技术研究”, 2016YFD0500804，2016-7至2021-6，65万，结题。

8. 扬州大学高层次人才启动经费"病原体的快速检测及应用研究"，137011016，2016-10至2020-09，100万，结题。

三、研究论文

1. Runbo Tian, Qihui Zhang, Haibing Zhu, Yaping Liu, Jing Jin, Zhanjun Yang* and Xin Zhou*. Phage-linked Immunosorbent Assay for Ultrasensitive Detection of Aβ42. Journal of Analysis and Testing. (2025). https://doi.org/10.1007/s41664-025-00399-2

2. Huquan Zhu, Shafiq Ur Rahman, Ting Pan, Jiasheng Yuan*, and Xin Zhou*. Dark-field intelligent detection of V. parahaemolyticus using T4 bacteriophage displaying tail spike proteins and gold nanoparticles. Biosensors & Bioelectronics, 2025 Jul 28;288: 117830.

3. Jincheng Zhang, Cuixing Zhan, Yue Sun, Ting Pan, Xin Zhou*. Exploring the efficacy of bacteriophage cocktails for mitigating Vibrio contamination within the seafood production chain: A feasible approach to microbial risk management. Food Control, 2025 November 177:111424.

4. Le He, Li Liu, Xin Zhou*, Zenglei Hu*, and Jiayin Shen*. Visual Counting of Influenza A Viruses with Magnetic T4 Phage SPR Probe，ACS Sensors，2025 10 (4), 2928-2937.

5. Ping Li, Wenjie Ma, Jun Cheng, Cuixing Zhan, Hongzhou Lu*, Jiayin Shen*, and Xin Zhou*. Phages adapt to recognize an O-antigen polysaccharide site by mutating the “backup” tail protein ORF59, enabling reinfection of phage-resistant Klebsiella pneumoniae. Emerging Microbes & Infections，2025, VOL. 14, 2455592.

6. Ping Li, Mangmang Shen, Haojie Ge, Xinan Jiao, Xin Zhou*, Xiang Chen*. Avi-tag mediated Phage@gold nanoprobe illuminates bacteria. Chemical Engineering Journal, 2024, 502, 157801. 

7. Jiasheng Yuan, Huquan Zhu, Shixinyi Li, Benjamin Thierry, Chih-Tsung Yang, Chen Zhang*, Xin Zhou*. Truncated M13 phage for smart detection of E. coli under dark field. Journal of Nanobiotechnology, 2024, 22, 599. 

8. Yukun Zeng, Ping Li, Shenglong Liu, Mangmang Shen, Yuqing Liu*, Xin Zhou*. Salmonella enteritidis acquires phage resistance through a point mutation in rfbD but loses some of its environmental adaptability. Veterinary Research, 2024, 55, 85.

9. Yukun Zeng, Mangmang Shen, Shenglong Liu, Xin Zhou*. Characterization and resistance mechanism of phage-resistant strains of Salmonella enteritidis. Poultry Science, 2024,103(6):103756. 

10. Jinxiu Hou, Xuejia Qian, Yi Xu, Zhirui Guo, Benjamin Thierry, Chih-Tsung Yang*, Xin Zhou*, Chuanbin Mao*. Rapid and reliable ultrasensitive detection of pathogenic H9N2 viruses through virus-binding phage nanofibers decorated with gold nanoparticles. Biosensors & Bioelectronics, 2023, 237, 115423. 

11. Jinxiu Hou, Yi Xu, Shasha Sun, Xiaoli Zhong, Chih-Tsung Yang*, Xin Zhou*. Gold nanoparticles-decorated M13 phage SPR probe for dual detection of antigen biomarkers in serum. Sensors & Actuators: B. Chemical, 2023,374,132811. 

12. Yuan Jiasheng, Shen Jiayin, Chen Mingyu, Lou Zhichao, Zhang Shuye, Song Zhigang, Li Weiwei*, Xin Zhou*. Artificial intelligence-assisted enumeration of ultra-small viruses with dual dark-field plasmon resonance probes. Biosensors & Bioelectronics, 2021,199,113893. 

13. Haixu Xu, Jiayin Shen, Chih-Tsung Yang, Benjamin Thierry, Ye. Zhu, Chuanbin Mao*, Xin Zhou*. Naked-eye counting of pathogenic viruses by phage-gold nanobiomaterials as probes. Materials Today Advances, 2021,10,100122. 

14. Chen G, Bai Y, Li Z, Wang F, Fan X, Zhou X*. Bacterial extracellular vesicle-coated multi-antigenic nanovaccines protect against drug-resistant Staphylococcus aureus infection by modulating antigen processing and presentation pathways. Theranostics, 2020, 10(16): 7131-7149. 

15. Fenglei Chen†, Tao Di†, Chih-Tsung Yang, Tianyu Zhang, Benjamin Thierry, and Xin Zhou*. “Naked-eye enumeration of single chlamydia pneumoniae based on light scattering of gold nanoparticle probe”. ACS Sensors, 2020, 5, 4, 1140-1148. 

16. Zhao Qian，Du, Cui，Jia, Yuanzheng, Yuan Jiasheng, Song Guangming, Zhou Xin*, Sun Shasha, Zhou Chen, Zhao Leipu, Yang Shengyang*. Solar-powered Janus membrane for one-step conversion of sewage to clean water. Chemical Engineering Journal, 2020, 387, 124131. 

17.   Xin Zhou*, Chih-Tsung Yang, Qiaoshu Xu, Zhichao Lou, Zhengfeng Xu, Benjamin Thierry, and Ning Gu. Gold Nanoparticle Probe-Assisted Antigen-Counting Chip Using SEM. ACS Appl. Mater. Interfaces, 2019, 11, 6769-6776. 

18. Fenglei Chen†, Fang Tang†, Chih-Tsung Yang, Xinyao Zhao, Jun Wang, Benjamin Thierry, Vipul Bansal, Jianjun Dai and Xin Zhou*. Fast and Highly Sensitive Detection of Pathogens Wreathed with Magnetic Nanoparticles Using Dark-Field Microscopy. ACS Sensors, 2018, 3, 2175-2181.

19.  Xin Zhou, Peng Cao, Ye Zhu, Wuguang Lu, Ning Gu*, Chuanbin Mao*. Phage-mediated counting by the naked eye of miRNA molecules at attomolar concentrations in a Petri dish. Nature Materials, 2015, 14(10): 1058–1064. 

20. Minfang Zhang*, Xin Zhou, Sumio Iijima, and Masako Yudasaka*, Small-Sized Carbon Nanohorns Enabling Cellular Uptake Control. Small, 2012, 8(16): 2524–2531.

21.  Xin Zhou, Sijing Xia, Zhiqiang Lu, Yuan Tian, Yishu Yan, Jin Zhu* Biomineralization-Assisted Ultrasensitive Detection of DNA, J. Am. Chem. Soc., 2010, 132 (20): 6932–6934. 

22.  Xin Zhou, Peng Cao, Yuan Tian, Jin Zhu*. Expressed Peptide Assay with Nanoparticle Probes, J. Am. Chem. Soc., 2010, 132 (12): 4161–4168. 

23. Min Hong, Xin Zhou, Zhiqiang Lu, Jin Zhu*. Nanoparticle-Based, Fluorous Tag-Driven DNA Detection, Angew Chem Int Ed., 2009, 48 (50):9503-9506.

四、授权国家发明专利

1. 周昕，侯金秀。M13噬菌体探针及其制备方法。专利号：ZL202110502844.6。申请日期：2021.5.8。授权日期：2023.8.25。

2. 周昕，袁嘉晟，张天宇。一种核酸抗体修饰的新型免疫金探针的制备。专利号：ZL201910268840.9。申请日期：2019-04-04。授权日期：2022.02.11。

3. 周昕，陈铭煜，袁嘉晟。基于深度学习分析暗场显微镜中纳米探针捕获病毒的方法。专利号：ZL202011297415.1。申请日期：2020.11.18。授权日期：2021.08.31。

4. 周昕，邸涛。一种基于金纳米探针鉴定单个肺炎衣原体的方法。专利号：ZL201810054094.9。申请日期：2018-01-19。授权日期：2021.03.09。

5. 周昕，李真真。磁性介孔二氧化硅纳米粒子探针及其制备方法和应用。专利号：ZL201910475019.4。申请日期：2019-05-31。授权日期：2020.09.29。