DOI number:10.3390/v13081606
Affiliation of Author(s):Yangzhou University
Journal:Viruses
Key Words:H9N2 avian influenza virus, HA protein, Newcastle disease virus, recombinant virus, thermostability, transmembrane domain
Abstract:H9N2 avian influenza virus (AIV) has become endemic in many countries, causing great economic losses when co-infected with other pathogens. So far, several live vaccines based on Newcastle disease virus (NDV) vectors expressing influenza hemagglutinin (HA) have been developed. However, the thermostable recombinant NDV is rarely reported. In this study, using a thermostable NDV rAHR09 strain as the vector, three recombinant NDVs expressing native HA, chimeric HA ectodomain with transmembrane domain/C-terminal cytoplasmic tail domain from fusion protein of NDV, and HA ectodomain were generated, designated rAHR09-HA, rAHR09-HAF, and rAHR09-HAE. The MDT value of three recombinant NDVs was above 120 h, their ICPI value was about 0.03, and the recombinant NDVs were still infectious when treated for 100 min under 56 degrees C, which demonstrated that the recombinant NDVs kept the lentogenic and thermostable nature of rAHR09. The immunization data showed that rAHR09-HA and rAHR09-HAF induced a higher HI antibody titer against H9N2 AIV and NDV. After being challenged with H9N2 AIV, the rAHR09-HA and rAHR09-HAF could significantly reduce the virus shedding in cloacal and tracheal swab samples. Our results suggest that rAHR09-HA and rAHR09-HAF might be vaccine candidates against H9N2 AIV.
Co-author:Zongyi Bo,chenchen Meng,Yin Chen,Chengcheng Zhang
First Author:Xiaorong Zhang
Indexed by:Research Atricle
Correspondence Author:Yongzhong Cao,Yantao Wu
Discipline:Agricultural science
First-Level Discipline:Veterinary Medicine
Document Type:J
Volume:13
Issue:8
Page Number:1606
ISSN No.:1999-4915
Translation or Not:no
Date of Publication:2021-08-29
Included Journals:SCI