DOI number:10.16303/j.cnki.1005-4545.2021.11.06
Affiliation of Author(s):Yangzhou University
Journal:Chinese journal of veterinary science
Key Words:avian infectious bronchitis virus; GVI-1 genotype; RT-qPCR; discriminant detection
Abstract:In order to rapidly distinguish the GVI-1 strains from other genotypes of avian infectious bronchitis virus (IBV), specific primers and probe were designed based on the S1 gene sequence alignment analysis among different genotypes to establish a real-time quantitative PCR (qPCR) assay. The results demonstrated that this assay showed good linearity within the concentration range tested, with a correlation coefficient of 0.999. The limit of detection was 1.0×10∧1 copies/µL. The assay is specific against GVI-1 strains and does not exhibit cross reactivity with other genotype strains such as the GI-1, GI-7, GI-13, GI-19 and GI-28. The variation coefficients of repeated test intra and inter group were both less than 2.5%. This assay and PCR were used to detect virus of oral and cloacal swabs, which were collected from infected chicks. The coincidence rate of the two methods was 96.7%, and the sensitivity of qPCR was higher. In conclusion, the assay for detection of GVI-1 genotype of IBV established in this study showed good specificity, sensitivity, and repeatability, which has important implications for GVI-1 genotype of IBV in epidemiological surveillance and rapid screening for clinical isolates.
Co-author:Xubin Du,Kunyan,Kai Liao,Chengcheng Zhang,Mengjiao Guo,Yantao Wu
First Author:Shuqin Chen
Indexed by:Research Atricle
Correspondence Author:Xiaorong Zhang
Document Code:1005-4545（2021）11-2121-05
Discipline:Agricultural science
First-Level Discipline:Veterinary Medicine
Document Type:J
Volume:41
Issue:11
Page Number:2121-2125, 2131
Translation or Not:no
Date of Publication:2021-11-15