Generation and Evaluation of Recombinant Thermostable Newcastle Disease Virus Expressing the HA of H9N2 Avian Influenza Virus
发布时间：2022-01-28点击次数：
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DOI码：10.3390/v13081606
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所属单位：扬州大学
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发表刊物：Viruses
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关键字：H9N2 avian influenza virus, HA protein, Newcastle disease virus, recombinant virus, thermostability, transmembrane domain
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摘要：H9N2 avian influenza virus (AIV) has become endemic in many countries, causing great economic losses when co-infected with other pathogens. So far, several live vaccines based on Newcastle disease virus (NDV) vectors expressing influenza hemagglutinin (HA) have been developed. However, the thermostable recombinant NDV is rarely reported. In this study, using a thermostable NDV rAHR09 strain as the vector, three recombinant NDVs expressing native HA, chimeric HA ectodomain with transmembrane domain/C-terminal cytoplasmic tail domain from fusion protein of NDV, and HA ectodomain were generated, designated rAHR09-HA, rAHR09-HAF, and rAHR09-HAE. The MDT value of three recombinant NDVs was above 120 h, their ICPI value was about 0.03, and the recombinant NDVs were still infectious when treated for 100 min under 56 degrees C, which demonstrated that the recombinant NDVs kept the lentogenic and thermostable nature of rAHR09. The immunization data showed that rAHR09-HA and rAHR09-HAF induced a higher HI antibody titer against H9N2 AIV and NDV. After being challenged with H9N2 AIV, the rAHR09-HA and rAHR09-HAF could significantly reduce the virus shedding in cloacal and tracheal swab samples. Our results suggest that rAHR09-HA and rAHR09-HAF might be vaccine candidates against H9N2 AIV.
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合写作者：薄宗义,孟晨晨,陈银,张成成
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第一作者：张小荣
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论文类型：Research Atricle
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通讯作者：曹永忠,吴艳涛
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学科门类：农学
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一级学科：兽医学
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文献类型：J
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卷号：13
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期号：8
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页面范围：1606
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ISSN号：1999-4915
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是否译文：否
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发表时间：2021-08-29
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收录刊物：SCI
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张小荣
个人信息
- 正高级
博士生导师
硕士生导师 - 教师拼音名称：Zhang Xiaorong
- 出生日期：1978-09-30
- 电子邮箱：
- 入职时间：2003-07-01
- 所在单位：兽医学院
- 学历：博士研究生毕业
- 办公地点：文汇路校区农牧南楼S203
- 性别：男
- 联系方式：E.mail:zxr@yzu.edu.cn
- 学位：博士
- 职称：正高级
- 在职信息：在岗
- 毕业院校：扬州大学
- 学科：预防兽医学
曾获荣誉：
- 2021-12-31扬州大学“十三五”科技工作先进个人
- 2021-10-222020-2021学年“最受学生欢迎的班主任”
- 2017-06-302016年江苏省普通高等学校本专科毕业设计（论文）三等奖指导教师
- 2016-01-182014-2015学年“最受学生欢迎的班主任”
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